Staining Toxoplasma Gondii with Fluorescein-labelled Antibody

نویسنده

  • Morris Goldman
چکیده

A new serologic test for antibodies to Toxoplasma is described, which is based upon inhibition of specific staining with fluorescent antibody. In performing the test, a mixture of the test serum and known fluorescein-labelled antiserum is added to a dried smear of toxoplasms for 1 hour at 37 degrees C. The smear is then rinsed and examined with a fluorescence microscope. Reduction in the brightness of fluorescence, as compared to that of a negative control slide, indicates the presence of antibody in the test serum. A comparison of the results of this test with those of the methylene blue dye test showed a strong parallelism between the two sets of results. On the other hand, the complement-fixation test for toxoplasmosis did not yield nearly as many positives as the inhibition test. The specificity of the new test was studied by comparing it with dye test results and clinical histories in human patients, and by testing a group of animals immunized with a variety of non-Toxoplasma antigens. No evidence of cross-reactions was obtained in the latter series. Some advantages and disadvantages of the inhibition test are discussed.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

STAINING TOXOPLASMA GONDII WITH FLUORESCEINo LABELLED ANTIBODY I. Tm~ REACTION IN SMEARS OF PERITONEAL EXUDATE BY MORRIS GOLDMAN, Sc.D

Toxoplasma gondii is a small organism which ordinarily is difficult to identify in tissue sections unless it occurs in clusters known as pseudocysts. A staining method specific for the organism would therefore be of value in the microscopic study of lesions suspected of being caused by this species. Since fluorescein-labelled antibody can be used to stain homologous organisms specifically (refe...

متن کامل

Toxoplasma gondii serosurvey in Golden Jackals from Golestan province, Iran

Toxoplasma gondii as an apicomplexan zoonotic parasite is able to infect humans and almost all other warm-blooded animals. Wild and domestic felids are the main definitive hosts. Also, canids such as dogs and jackals have been introduced as mechanical vectors since T. gondii oocysts can pass their intestinal tract and spread by feces in environment. Toxoplasmosis is a common zoonotic disease in...

متن کامل

Latex agglutination test for detection of antibodies to Toxoplasma gondii.

A resurgence of interest in Toxoplasma gondii has occurred because this coccidian parasite causes lethal infections in immunologically compromised hosts and is responsible for at least 3,000 congenitally infected infants in the United States annually. Thus, rapid, specific, and inexpensive serologic tests are required for routine screening of patients, especially pregnant women. We have develop...

متن کامل

Coinfection of Toxoplasma Gondii and Intestinal Parasites among Elderly Immunocompromised Patients

Introduction: Patients with cancer and hemodialysis are prone to opportunistic infections. The present research aims to examine the frequency of Toxoplasmosis and intestinal parasites in these patients. Methods: In the present cross-sectional study, seventy stool and blood samples from immunocompromised patients (46 hemodialysis and 24 cancer patients) from Guilan Province were collected in 20...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of Experimental Medicine

دوره 105  شماره 

صفحات  -

تاریخ انتشار 1957